Coding

Part:BBa_K1683003:Design

Designed by: Matthew W Mortensen   Group: iGEM15_WLC-Milwaukee   (2015-09-17)


E. coli tolC signaling sequence + Proteus mirabilis tolC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1331
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 876
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The main consideration when designing the signaling sequence and Proteus mirabilis TolC gene was to use silent mutations alter any RFC10 incompatible DNA sections to be biobrick compatible.


Source

The tolC signaling sequence and Proteus mirabilis tolC sequence were synthesized from DNA sequences found in genomic databases

References